Strain Name	NOG-FcgR KO (NOGF)

• You will be required to sign a consent form upon purchase.
• The use of this mouse strain corresponds to the use of Living Modified Organism (Cartagene Protocol, domestic law). We will send you an information provision form in advance, so please check it and use it according to the rules of each institution.

Development

Frozen embryos of NOD.Cg-Fcer1g<tm1Rav>Fcgr2b<tm1Ttk> established by Dr. Toshiyuki Takai (Professor, Department of Experimental Immunology, Institute of Development, Aging and Cancer, Tohoku University) were obtained from RIKEN BRC (RBRC02330, Tsukuba, Japan).
Mouse restored from these frozen embryos was backcrossed with NOG mouse to introduce the scid mutation and the IL-2Rγc target allele and establish them.

Product Features

• This strain is deleted FcgR gene of NOG mouse.
• Innate immunity in mouse is attenuated.
• The anti-tumor effects of immune checkpoint inhibitory antibodies in humanized NOGF mouse engrafted with human hematopoietic stem cells can be clearly evaluated compared to conventional NOG mouse.

Research Applications

• Experiment to evaluate the antitumor effect of immune checkpoint inhibitors using HSC-engrafted humanized NOGF mouse.
• Evaluation of anti-tumor effect by combined therapy with CAR-T cells etc. and immune checkpoint inhibitors etc.

• Human hematopoietic stem cell (HSC) engrafted models are commonly used.
• We produce humanized NOGF mouse in Japan and deliver them to customers.
• Production will be started after receiving your firm order. We usually ship about 12 weeks after an order is placed.
• You will be required to sign a consent form upon purchase.
• The use of this mouse strain corresponds to the use of Living Modified Organism (Cartagene Protocol, domestic law). We will send you an information provision form in advance, so please check it and use it according to the rules of each institution.

Product features of humanized NOGF mouse engrafted with Hematopoietic Stem Cells (HSC)

• In HSC engrafted humanized NOGF mouse, stem cells mainly differentiate and engraft into human T cells and human B cells. Some are also differentiated into NK cells, macrophages, and monocytes, but they are very few.
• Compared to conventional humanized NOG mouse, the chimeric ratio of human CD45 positive cells in peripheral blood tends to be higher.
• Analysis of human cell subsets also revealed that humanized NOGF mouse had a significantly higher proportion of CD19 positive B cells and a lower proportion of CD3 positive T cells, CD33 positive myeloid cells and CD56 positive NK cells 16 weeks after HSC engraftment. (Katano et al. Scientific reports 2021)
• Graft versus host disease (GVHD) caused by human immune cells is not usually seen. It has been confirmed that in many cases, even if cancer cells are transplanted after engraftment of human immune cells, they will not be rejected and to be engrafted.
• Differentiated human T cells have been confirmed to be activated by drugs and cytokines and have cytotoxic activity against cancer cells.
• In the US and EU countries, TACONIC Biosciences supplies humanized NOG-EXL mouse. The quality standard is the same as that of humanized NOG-EXL mouse supplied in Japan.
• We are providing detailed briefings by online regarding the products features of humanized NOG-EXL mouse, the production of humanized NOG-EXL mouse, and experimental protocols for their use. Please feel free to contact us at sales@invivoscience.com.

Back Ground Data

• Cell differentiation in peripheral blood after HSC transfer in NOGF mouse. Comparison of humanized NOGF and humanized NOG mouse.
• Antitumor effects of humanized NOGF mouse used nivolumab. Comparison of humanized NOGF and humanized NOG mouse.

Video: NOGF and humanized NOGF

Introducing the establishment, features, and major research applications of NOGF mouse.
An anti-tumor evaluation of an immune checkpoint inhibitor (nivolumab) in huNOGF mouse engrafted with hematopoietic stem cells is introduced.